There have now been 60 cases of Covid-19 confirmed in Scotland – a rise of 24 from Wednesday.
Economy Secretary Fiona Hyslop said the situation presents a “significant risk” to the global and domestic economies, which is why there was a need for the new business helpline.
She said that work was under way to identify further needs of businesses including calls for planning authorities to relax their approach to restrictions on shop delivery times and opening hours.
Ms Hyslop said: “I also chaired a meeting with representatives from Scotland’s business support organisations including the STUC to hear their concerns and provide reassurance that the Scottish government will do everything in our power to protect the workforce, businesses and our economy.
“I’d also encourage everyone to refer to the new guidance on Covid-19 that includes workplace advice on reducing the spread of infection.
“Following the UK government’s budget we also require urgent clarification on what funding Scotland will receive from the announcements made by the UK government, at a time when prospects for the economy and public finances remain very uncertain as the short term impacts of Covid-19 unfold.”